Development of Colorimetric Microtiter Plate Assay for Assessment of Antimicrobials against Acanthamoeba
نویسندگان
چکیده
منابع مشابه
Development of colorimetric microtiter plate assay for assessment of antimicrobials against Acanthamoeba.
We have developed and optimized a 96-well microtiter plate assay, based on the reduction of alamarBlue, to assess the efficacies of much needed new antimicrobials against Acanthamoeba species. This assay has been optimized for determination of drug efficacy against two potentially pathogenic species, Acanthamoeba castellanii and Acanthamoeba polyphaga, and has been validated by comparison of th...
متن کاملSimple colorimetric microtiter plate hybridization assay for detection of amplified Mycobacterium leprae DNA.
The detection of amplified products resulting from polymerase chain reactions (PCRs) remains a complicated process. To simplify the detection procedures, we developed a colorimetric microtiter plate hybridization assay for the specific detection of 5'-biotinylated PCR fragments of Mycobacterium leprae DNA. For this assay, an M. leprae DNA capture probe was made and immobilized on the wells of a...
متن کاملMicrotiter plate immunoenzymometric assay for estrogen receptor.
The estrogen receptor (ER) status of breast cancer is used both as a prognostic factor and as a predictor of response to endocrine therapy. An immunoenzymometric assay for ER was developed on 96-well microtiter plates (EIA96). This technique involves two monoclonal antibodies directed against different epitopes in the B domain of ER. The two-step protocol (16-18 h and 3 h at 4 degrees C) requir...
متن کاملA Microtiter Plate-Based Assay for Inhibitors of Penicillin-Binding
PBP2a, the molecular determinant for high level β-lactam resistance in 19 Methicillin-resistant Staphylococcus aureus (MRSA), is intrinsically resistant to most β20 lactam antibiotics. The development and characterization of new inhibitors targeting 21 PBP2a would benefit from an effective and convenient assay for inhibitor binding. This 22 study was directed towards the development of a fluore...
متن کاملdiagnostic and developmental potentials of dynamic assessment for writing skill
این پایان نامه بدنبال بررسی کاربرد ارزیابی مستمر در یک محیط یادگیری زبان دوم از طریق طرح چهار سوال تحقیق زیر بود: (1) درک توانایی های فراگیران زمانیکه که از طریق برآورد عملکرد مستقل آنها امکان پذیر نباشد اما در طول جلسات ارزیابی مستمر مشخص شوند; (2) امکان تقویت توانایی های فراگیران از طریق ارزیابی مستمر; (3) سودمندی ارزیابی مستمر در هدایت آموزش فردی به سمتی که به منطقه ی تقریبی رشد افراد حساس ا...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2005
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.43.2.629-634.2005